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This report presents results of independent laboratory tests conducted on the effectiveness of ozone against five strains of microorganisms. The test subjects were chosen because they represent common problems in our modern living. Three bacteria were chosen (one especially for its hardiness and resistance to chemicals) as well as one yeast, Candida, and one mold, Aspergillus.
The tests were conducted using one of our air purifiers which produced about .05 ppm of ozone. This is the limit below which the FDA does not recognize ozone production. If we had chosen a lower level the results would have been the same with perhaps the 1/2 hour readings not quite as
dramatic.
From the data and the graphical representations it can be seen
that dramatic, quick and effective destruction of all of the
test subjects was accomplished. Note the 1/2 hour results. Note
also that after some time, if total destruction is not achieved,
the organisms are able to re-establish themselves. This shows
the necessity of continuous use of the air purifier.
SUMMARY:
From the results of the tests, after four hours' exposure to ozone,
present between 0.049 and 0.064 parts per million generated by the air
purifier, numbers of all five tested microorganisms were significantly
reduced on the test surfaces when compared to control surfaces exposed to
normal room air.
METHODOLOGY:
A containment chamber constructed from a polyethylene glove bag was
used for the ozone exposure. Once the chamber atmosphere reached a
relatively stable 0.05 parts per million ozone concentration, stainless
steel Penicillin ring carriers were coated with 48 hour culture
suspensions and dried for 30 minutes at 35°C. Test carriers were exposed
to ozone for 0, ˝, 1, 2, and 4 hours intervals. Control carriers were
exposed to normal room air for 4 hours. After their respective exposures,
each carrier was immersed in a tube of buffered ozone-neutralizing
solution. These tubes were held in an ice bath in the chamber. The ice
bath also served to maintain a high humidity. At the end of the test
period, the chamber was opened, and the control carriers tubes added to
the ice bath until quantification. Quantification was performed by serial
dilution and plating on Lactose agar for the bacteria, and on Mycological
agar for the fungi (mold). Adapted from AOAC Method 4.010.
The chamber ozone concentration was determined by the titration of the
ozone impinged in a potassium iodide solution by a calibrated air pump per
Standard Methods, Method 422.
Please click on Thumbnails for details.
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| Staphylococcus |
Salmonella |
E-coli |
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| Candida |
Aspergillus |
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Our whole
home air purifiers are designed to produce .02 to .04
ppm of ozone when set for 24 hour a day use. The air purifiers
can also be set at higher levels to sanitize an unoccupied home
quickly. The air purifiers use both ozone as well as a proprietary
ionizer to keep the air clean in an entire home. Ozone destroys
microorganisms such as mold and bacteria while ionization removes them from our
breathing space. |